Journal: Pharmaceuticals

Article Title: Shenqi Granules Enhance Recovery from Myocardial Ischemia–Reperfusion Injury by Downregulating MMP9 and ADH1C

doi: 10.3390/ph19030475

Figure Lengend Snippet: SQG targets were screened for the treatment of MIRI. ( A ) Venn diagram for the intersectional genes from the hub gene of yellow module, SQG targets, GeneCards, DisGeNET, and DEGs. ( B ) The expression analysis of MMP9 and ADH1C in GSE62646 dataset. ( C , D ) The ROC analysis of MMP9 ( C ) and ADH1C ( D ).

Article Snippet: Primary antibodies used were against MMP9 (1:1000, Proteintech, Wuhan, China), ADH1C (1:1000, Proteintech, China), and GAPDH (1:2000, Proteintech, China).

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Shenqi Granules Enhance Recovery from Myocardial Ischemia–Reperfusion Injury by Downregulating MMP9 and ADH1C

doi: 10.3390/ph19030475

Figure Lengend Snippet: MMP9 and ADH1C play important roles in immune infiltration of MIRI. ( A , B ) The differences in immune cell infiltration between patients and controls were analyzed using CIBERSORT ( A ) and Xcell ( B ) algorithms. ( C ) Correlation analysis between immune cells and MMP9 or ADH1C. ( D ) Predictive analysis of MMP9 protein and ADH1C protein interaction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared with the control group.

Article Snippet: Primary antibodies used were against MMP9 (1:1000, Proteintech, Wuhan, China), ADH1C (1:1000, Proteintech, China), and GAPDH (1:2000, Proteintech, China).

Techniques: Control

Journal: Pharmaceuticals

Article Title: Shenqi Granules Enhance Recovery from Myocardial Ischemia–Reperfusion Injury by Downregulating MMP9 and ADH1C

doi: 10.3390/ph19030475

Figure Lengend Snippet: MMP9 and ADH1C are the key targets of SQG improving MIRI. ( A ) Heatmap of binding energies between six SQG components and MMP9/ADH1C. ( B ) Molecular docking: MMP9-luteolin and ADH1C-stigmasterol. ( C – G ) Molecular dynamics of the MMP9-luteolin complex: system RMSD ( C ), RMSF ( D ), Rg ( E ), SASA ( F ), and hydrogen bonds ( G ). ( H – L ) Molecular dynamics of the ADH1C-stigmasterol complex: system RMSD ( H ), RMSF ( I ), Rg ( J ), SASA ( K ), and hydrogen bonds ( L ). ( M ) Western blot results for MIRI treatment with SQG, n = 3 rats per group. Data are presented as mean ± SEM of three independent experiments. ## p < 0.01, ### p < 0.001, compared with the Sham group. * p < 0.05, ** p < 0.01, compared with the I/R model group.

Article Snippet: Primary antibodies used were against MMP9 (1:1000, Proteintech, Wuhan, China), ADH1C (1:1000, Proteintech, China), and GAPDH (1:2000, Proteintech, China).

Techniques: Binding Assay, Western Blot

Journal: Alcoholism, clinical and experimental research

Article Title: Preservation of hepatocyte nuclear factor-4α contributes to the beneficial effect of dietary medium chain triglyceride on alcohol-induced hepatic lipid dyshomeostasis in rats.

doi: 10.1111/acer.12013

Figure Lengend Snippet: Fig. 1. Effects of alcohol and medium chain triglyceride (MCT) on alcohol metabolism. (A) Immunoblot analysis of hepatic alcohol dehydrogenase (ADH) and CYP2E1 protein and band intensity of blots. (B) Blood alcohol concentrations. Data are expressed as mean ± SD (n = 3 in A; n = 6 to 8 in B). Statistical differences were analyzed by ANOVA followed by Student-Newman–Keuls test, and means without a common letter differ at p < 0.05.

Article Snippet: The membrane was probed with rabbit polyclonal or mouse monoclonal antibodies against CYP2E1 from Calbiochem (San Diego, CA), ADH (alcohol dehydrogenase) and HNF4a from Novus Biotechnologies (Littleton, CO), and ApoB100 (apolipoprotein B 100), ACADM (acyl-CoA dehydrogenase, medium chain), ACADL (acyl-CoA dehydrogenase, long chain), MTTP (microsomal triglyceride transfer protein), HNF1a (hepatocyte nuclear factor-1a), PPARa (peroxisome proliferator-activated receptor a), and C/EBPa (CCAAT/enhancer-binding protein a) from Santa Cruz Biotechnologies (Santa Cruz, CA), followed by incubation with HRP-conjugated goat anti-rabbit IgG, or goat anti-mouse IgG antibody.

Techniques: Western Blot